| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ANGPT1 (280-498) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ANGPT1 |
| Gene ID: |
284 |
| Uniprot ID: |
ANGP1_HUMAN |
| Immunogen Region: |
280-498 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 280-498 of human ANGPT1 (NP_001137.2). |
| Immunogen Sequence: |
EKPFRDCADVYQAGFNKSGI YTIYINNMPEPKKVFCNMDV NGGGWTVIQHREDGSLDFQR GWKEYKMGFGNPSGEYWLGN EFIFAITSQRQYMLRIELMD WEGNRAYSQYDRFHIGNEKQ NYRLYLKGHTGTAGKQSSLI LHGADFSTKDADNDNCMCKC ALMLTGGWWFDACGPSNLNG MFYTAGQNHGKLNGIKWHYF KGPSYSLRSTTMMIRPLDF |
| Post Translational Modifications | Glycosylated. |
| Function | Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. |
| Protein Name | Angiopoietin-1Ang-1 |
| Database Links | Reactome: R-HSA-210993Reactome: R-HSA-5673001 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Angiopoietin-1 antibodyAnti-Ang-1 antibodyAnti-ANGPT1 antibodyAnti-KIAA0003 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
