• Western blot analysis of extracts of various cell lines, using AMY2A antibody (STJ110348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of paraffin-embedded human pancreas using AMY2A rabbit polyclonal antibody (STJ110348) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded mouse pancreas using AMY2A rabbit polyclonal antibody (STJ110348) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat pancreas using AMY2A rabbit polyclonal antibody (STJ110348) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AMY2A antibody (16-511) (STJ110348)

SKU:
STJ110348

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AMY2A (16-511) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AMY2A
Gene ID: 279
Uniprot ID: AMYP_HUMAN
Immunogen Region: 16-511
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 16-511 of human AMY2A (NP_000690.1).
Immunogen Sequence: QYSPNTQQGRTSIVHLFEWR WVDIALECERYLAPKGFGGV QVSPPNENVAIYNPFRPWWE RYQPVSYKLCTRSGNEDEFR NMVTRCNNVGVRIYVDAVIN HMCGNAVSAGTSSTCGSYFN PGSRDFPAVPYSGWDFNDGK CKTGSGDIENYNDATQVRDC RLTGLLDLALEKDYVRSKIA EYMNHLIDIGVAGFRLDASK HMWPGDIKAILDKLHNLNSN WFPAGSKPFIYQEVIDLGG
Tissue Specificity Detected in pancreas (at protein level).
Protein Name Pancreatic Alpha-Amylase
Pa
1 -4-Alpha-D-Glucan Glucanohydrolase
Database Links Reactome: R-HSA-189085
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Pancreatic Alpha-Amylase antibody
Anti-Pa antibody
Anti-1 -4-Alpha-D-Glucan Glucanohydrolase antibody
Anti-AMY2A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance