Anti-AMOT antibody (710-790 aa) (STJ190548)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 710-790
General Information
| Short Description | Rabbit polyclonal anti-Angiomotin (710-790 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AMOT |
| Gene ID | 154796 |
| Uniprot ID | AMOT_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 710-790 |
| Immunogen Region | 710-790 aa |
| Specificity | AMOT Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation. |
| Function | Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Repressor of YAP1 and WWTR1/TAZ transcription of target genes, potentially via regulation of Hippo signaling-mediated phosphorylation of YAP1 which results in its recruitment to tight junctions. |
| Protein Name | Angiomotin |
| Database Links | Reactome: R-HSA-2028269 Q4VCS5-1Reactome: R-HSA-9762292 Q4VCS5-2 |
| Cellular Localisation | Cell JunctionTight JunctionLocalized On The Cell SurfaceMay Act As A Transmembrane Protein |
| Alternative Antibody Names | Anti-Angiomotin antibodyAnti-AMOT antibodyAnti-KIAA1071 antibody |
Information sourced from Uniprot.org