• Immunofluorescence analysis of mouse kidney using AMACR Rabbit polyclonal antibody (STJ22599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat kidney using AMACR Rabbit polyclonal antibody (STJ22599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human prostate cancer using AMACR Rabbit polyclonal antibody (STJ22599) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using AMACR antibody (STJ22599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AMACR antibody (1-138) (STJ22599)

SKU:
STJ22599

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AMACR (1-138) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AMACR
Gene ID: 23600
Uniprot ID: AMACR_HUMAN
Immunogen Region: 1-138
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human AMACR (NP_055139.4).
Immunogen Sequence: MALQGISVVELSGLAPGPFC AMVLADFGARVVRVDRPGSR YDVSRLGRGKRSLVLDLKQP RGAAVLRRLCKRSDVLLEPF RRGVMEKLQLGPEILQRENP RLIYARLSGFGQSGSFCRLA GHDINYLALSGVLSKIGR
Function Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters. Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs.
Protein Name Alpha-Methylacyl-Coa Racemase
2-Methylacyl-Coa Racemase
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-389887
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Mitochondrion
Alternative Antibody Names Anti-Alpha-Methylacyl-Coa Racemase antibody
Anti-2-Methylacyl-Coa Racemase antibody
Anti-AMACR antibody

Information sourced from Uniprot.org

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