| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ALKBH1 (110-389) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IP 1:20-1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALKBH1 |
| Gene ID: |
8846 |
| Uniprot ID: |
ALKB1_HUMAN |
| Immunogen Region: |
110-389 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2). |
| Immunogen Sequence: |
YQWHWVKQCLKLYSQKPNVC NLDKHMSKEETQDLWEQSKE FLRYKEATKRRPRSLLEKLR WVTVGYHYNWDSKKYSADHY TPFPSDLGFLSEQVAAACGF EDFRAEAGILNYYRLDSTLG IHVDRSELDHSKPLLSFSFG QSAIFLLGGLQRDEAPTAMF MHSGDIMIMSGFSRLLNHAV PRVLPNPEGEGLPHCLEAPL PAVLPRDSMVEPCSMEDWQV CASYLKTARVNMTVRQVLA |
| Tissue Specificity | Ubiquitous. |
| Function | Dioxygenase that acts as on nucleic acids, such as DNA and tRNA. Requires molecular oxygen, alpha-ketoglutarate and iron. A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment. Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs. Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation. In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation. Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA. N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing. Demethylates mRNAs containing N(3)-methylcytidine modification. Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity. Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product. DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation. |
| Protein Name | Nucleic Acid Dioxygenase Alkbh1Alkylated Dna Repair Protein Alkb Homolog 1Alpha-Ketoglutarate-Dependent Dioxygenase Abh1Dna 6ma DemethylaseDna N6-Methyl Adenine Demethylase Alkbh1Dna Lyase Abh1Dna Oxidative Demethylase Alkbh1Mrna N(3-Methylcytidine Demethylase |
| Cellular Localisation | NucleusMitochondrionMainly Localizes In EuchromatinLargely Excluded From Heterochromatin And Nucleoli |
| Alternative Antibody Names | Anti-Nucleic Acid Dioxygenase Alkbh1 antibodyAnti-Alkylated Dna Repair Protein Alkb Homolog 1 antibodyAnti-Alpha-Ketoglutarate-Dependent Dioxygenase Abh1 antibodyAnti-Dna 6ma Demethylase antibodyAnti-Dna N6-Methyl Adenine Demethylase Alkbh1 antibodyAnti-Dna Lyase Abh1 antibodyAnti-Dna Oxidative Demethylase Alkbh1 antibodyAnti-Mrna N(3-Methylcytidine Demethylase antibodyAnti-ALKBH1 antibodyAnti-ABH antibodyAnti-ABH1 antibodyAnti-ALKBH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
