• Immunofluorescence analysis of L929 cells using ALG5 Rabbit polyclonal antibody (STJ119736) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ALG5 in paraffin-embedded Human colon carcinoma using ALG5 Rabbit polyclonal antibody (STJ119736) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ALG5 in paraffin-embedded Rat ovary using ALG5 Rabbit polyclonal antibody (STJ119736) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ALG5 in paraffin-embedded Mouse pancreas using ALG5 Rabbit polyclonal antibody (STJ119736) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ALG5 Rabbit polyclonal antibody (STJ119736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALG5 antibody (34-190) (STJ119736)

SKU:
STJ119736

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALG5 (34-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALG5
Gene ID: 29880
Uniprot ID: ALG5_HUMAN
Immunogen Region: 34-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-190 of human ALG5 (NP_037470.1).
Immunogen Sequence: MPALHRHEEEKFFLNAKGQK ETLPSIWDSPTKQLSVVVPS YNEEKRLPVMMDEALSYLEK RQKRDPAFTYEVIVVDDGSK DQTSKVAFKYCQKYGSDKVR VITLVKNRGKGGAIRMGIFS SRGEKILMADADGATKFPDV EKLEKGLNDLQPWPNQM
Tissue Specificity Expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung.
Function Required for the assembly of lipid-linked oligosaccharides in kidney epithelial cells, and protein N-glycosylation. Required for polycystin-1 (PKD1) glycosylation and maturation.
Protein Name Dolichyl-Phosphate Beta-Glucosyltransferase
Dolp-Glucosyltransferase
Asparagine-Linked Glycosylation Protein 5 Homolog
Database Links Reactome: R-HSA-480985
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Dolichyl-Phosphate Beta-Glucosyltransferase antibody
Anti-Dolp-Glucosyltransferase antibody
Anti-Asparagine-Linked Glycosylation Protein 5 Homolog antibody
Anti-ALG5 antibody
Anti-HSPC149 antibody

Information sourced from Uniprot.org

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