| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALG13 |
| Gene ID: |
79868 |
| Uniprot ID: |
ALG13_HUMAN |
| Immunogen Region: |
100-167 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 100-167 of human ALG13 (NP_001311219.1). |
| Immunogen Sequence: |
VVINEKLMNNHQLELAKQLH KEGHLFYCTCSTLPGLLQSM DLSTLKCYPPGQPEKFSAFL DKVVGLQK |
| Function | Catalytic subunit of the UDP-N-acetylglucosamine transferase complex that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. On the cytoplasmic face of the endoplasmic reticulum, the dimeric ALG13/ALG14 complex catalyzes the second step of dolichol pyrophosphate biosynthesis, transferring a beta1,4-linked N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to GlcNAc-pyrophosphatedolichol (Gn-PDol) to produce N,N'-diacetylchitobiosyl diphosphodolichol. N,N'-diacetylchitobiosyl diphosphodolichol is a substrate for ALG1, the following enzyme in the biosynthetic pathway. Isoform 2: Catalytic subunit of the UDP-N-acetylglucosamine transferase complex that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. On the cytoplasmic face of the endoplasmic reticulum, the dimeric ALG13/ALG14 complex catalyzes the second step of dolichol pyrophosphate biosynthesis, transferring a beta1,4-linked N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to GlcNAc-pyrophosphatedolichol (Gn-PDol) to produce N,N'-diacetylchitobiosyl diphosphodolichol. N,N'-diacetylchitobiosyl diphosphodolichol is a substrate for ALG1, the following enzyme in the biosynthetic pathway. Isoform 1: No glycosyltransferase or deubiquitinase activity is detected for this potential multifunctional enzyme. |
| Protein Name | Udp-N-Acetylglucosamine Transferase Subunit Alg13Asparagine-Linked Glycosylation 13 HomologGlycosyltransferase 28 Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-446193Reactome: R-HSA-5633231 |
| Cellular Localisation | Isoform 2: Endoplasmic Reticulum MembranePeripheral Membrane ProteinRecruited To The Cytosolic Face Of The Endoplasmic Reticulum Membrane Through Its Interaction With Alg14 |
| Alternative Antibody Names | Anti-Udp-N-Acetylglucosamine Transferase Subunit Alg13 antibodyAnti-Asparagine-Linked Glycosylation 13 Homolog antibodyAnti-Glycosyltransferase 28 Domain-Containing Protein 1 antibodyAnti-ALG13 antibodyAnti-CXorf45 antibodyAnti-GLT28D1 antibodyAnti-MDS031 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
