| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ALDH3A2 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration, 2-4µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ALDH3A2 |
| Gene ID: | 224 |
| Uniprot ID: | AL3A2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001026976.1; NP_000373.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_001026976.1; NP_000373.1). |
| Immunogen Sequence: | SLKREGANKLRYPP |
| Function | Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. |
| Protein Name | Aldehyde Dehydrogenase Family 3 Member A2Aldehyde Dehydrogenase 10Fatty Aldehyde DehydrogenaseMicrosomal Aldehyde Dehydrogenase |
| Database Links | Reactome: R-HSA-389599 P51648-2Reactome: R-HSA-9603798 P51648-2Reactome: R-HSA-9609523 P51648-1Reactome: R-HSA-9696270Reactome: R-HSA-9696273Reactome: R-HSA-9845614 P51648-1 |
| Cellular Localisation | Microsome MembraneSingle-Pass Membrane ProteinEndoplasmic Reticulum MembraneCytoplasmic Side |
| Alternative Antibody Names | Anti-Aldehyde Dehydrogenase Family 3 Member A2 antibodyAnti-Aldehyde Dehydrogenase 10 antibodyAnti-Fatty Aldehyde Dehydrogenase antibodyAnti-Microsomal Aldehyde Dehydrogenase antibodyAnti-ALDH3A2 antibodyAnti-ALDH10 antibodyAnti-FALDH antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit polyclonal antibody (STJ11100775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89711/358193/STJ11100775_1__25485.1713122509.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit polyclonal antibody (STJ11100775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
