• Western blot analysis of extracts of A-549 cells, using ALDH3A1 antibody (STJ27453) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of Rat stomach using ALDH3A1 antibody (STJ27453) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells using ALDH3A1 antibody (STJ27453) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using ALDH3A1 antibody (STJ27453) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse stomach using ALDH3A1 antibody (STJ27453) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALDH3A1 antibody (1-300) (STJ27453)

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STJ27453

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDH3A1 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH3A1
Gene ID: 218
Uniprot ID: AL3A1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ALDH3A1 (NP_001128640.1).
Immunogen Sequence: MSKISEAVKRARAAFSSGRT RPLQFRIQQLEALQRLIQEQ EQELVGALAADLHKNEWNAY YEEVVYVLEEIEYMIQKLPE WAADEPVEKTPQTQQDELYI HSEPLGVVLVIGTWNYPFNL TIQPMVGAIAAGNSVVLKPS ELSENMASLLATIIPQYLDK DLYPVINGGVPETTELLKER FDHILYTGSTGVGKIIMTAA AKHLTPVTLELGGKSPCYVD KNCDLDVACRRIAWGKFMN
Tissue Specificity High levels in stomach, esophagus and lung.low level in the liver and kidney.
Function ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids. Preferentially oxidizes aromatic aldehyde substrates. Comprises about 50 percent of corneal epithelial soluble proteins. May play a role in preventing corneal damage caused by ultraviolet light.
Protein Name Aldehyde Dehydrogenase - Dimeric Nadp-Preferring
Aldhiii
Aldehyde Dehydrogenase 3
Aldehyde Dehydrogenase Family 3 Member A1
Database Links Reactome: R-HSA-211945
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Aldehyde Dehydrogenase - Dimeric Nadp-Preferring antibody
Anti-Aldhiii antibody
Anti-Aldehyde Dehydrogenase 3 antibody
Anti-Aldehyde Dehydrogenase Family 3 Member A1 antibody
Anti-ALDH3A1 antibody
Anti-ALDH3 antibody

Information sourced from Uniprot.org

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