| Host: | Mouse |
| Applications: | ELISA/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-ALCAM (125-175) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Monoclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5-1.5 Mu g/Mu l |
| Dilution Range: | ELISA 1:10, 000 IP 1:200 WB 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | ALCAM |
| Gene ID: | 214 |
| Uniprot ID: | CD166_HUMAN |
| Immunogen Region: | 125-175 |
| Immunogen: | Synthetic peptide corresponding to amino acids within region 125-175 on human ALCAM protein. |
| Tissue Specificity | Detected on hematopoietic stem cells derived from umbilical cord blood. Detected on lymph vessel endothelial cells, skin and tonsil. Detected on peripheral blood monocytes. Detected on monocyte-derived dendritic cells (at protein level). Detected at low levels in spleen, placenta, liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines. |
| Post Translational Modifications | Glycosylated. |
| Function | Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation. Required for normal hematopoietic stem cell engraftment in the bone marrow. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis.required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation. Promotes neurite extension, axon growth and axon guidance.axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons. Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation. |
| Protein Name | Cd166 Antigen Activated Leukocyte Cell Adhesion Molecule Cd Antigen Cd166 |
| Database Links | Reactome: R-HSA-373760 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Axon Dendrite Detected At The Immunological Synapse I E At The Contact Zone Between Antigen-Presenting Dendritic Cells And T-Cells Colocalizes With Cd6 And The Tcr/Cd3 Complex At The Immunological Synapse Isoform 3: Secreted |
| Alternative Antibody Names | Anti-Cd166 Antigen antibody Anti-Activated Leukocyte Cell Adhesion Molecule antibody Anti-Cd Antigen Cd166 antibody Anti-ALCAM antibody Anti-MEMD antibody |
Information sourced from Uniprot.org