• Western blot analysis of various lysates, using Albumin antibody (STJ11104984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 75s.

Anti-Albumin antibody (25-607 aa) (STJ11104984)

SKU:
STJ11104984

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 25-607 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 25-607 of bovine Albumin. bovine Albumin. (NP_851335.1).
Immunogen Sequence: DTHKSEIAHRFKDLGEEHFK GLVLIAFSQYLQQCPFDEHV KLVNELTEFAKTCVADESHA GCEKSLHTLFGDELCKVASL RETYGDMADCCEKQEPERNE CFLSHKDDSPDLPKLKPDPN TLCDEFKADEKKFWGKYLYE IARRHPYFYAPELLYYANKY NGVFQECCQAEDKGACLLPK IETMREKVLTSSARQRLRCA SIQKFGERALKAWSVARLSQ KFPKAEFVEVTKLVTDLTK
Background Binds water, Ca (2+, Na (+, K (+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable. The rank order of affinity is zinc > calcium > magnesium (Probable. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance