• Immunofluorescence analysis of HeLa cells using ALAD antibody (STJ114271) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ALAD antibody (STJ114271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALAD antibody (1-330) (STJ114271)

SKU:
STJ114271

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALAD (1-330) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALAD
Gene ID: 210
Uniprot ID: HEM2_HUMAN
Immunogen Region: 1-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ALAD (NP_000022.3).
Immunogen Sequence: MQPQSVLHSGYFHPLLRAWQ TATTTLNASNLIYPIFVTDV PDDIQPITSLPGVARYGVKR LEEMLRPLVEEGLRCVLIFG VPSRVPKDERGSAADSEESP AIEAIHLLRKTFPNLLVACD VCLCPYTSHGHCGLLSENGA FRAEESRQRLAEVALAYAKA GCQVVAPSDMMDGRVEAIKE ALMAHGLGNRVSVMSYSAKF ASCFYGPFRDAAKSSPAFGD RRCYQLPPGARGLALRAVD
Function Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
Protein Name Delta-Aminolevulinic Acid Dehydratase
Aladh
Porphobilinogen Synthase
Database Links Reactome: R-HSA-189451
Reactome: R-HSA-6798695
Alternative Antibody Names Anti-Delta-Aminolevulinic Acid Dehydratase antibody
Anti-Aladh antibody
Anti-Porphobilinogen Synthase antibody
Anti-ALAD antibody

Information sourced from Uniprot.org

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