• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Akt1 S1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from 293 cells, using Akt1 S1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PRAS40 Polyclonal Antibody

Anti-AKT1S1 antibody (207-256 aa) (STJ95215)

SKU:
STJ95215

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Proline-rich AKT1 substrate 1 (207-256 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKT1S1
Gene ID: 84335
Uniprot ID: AKTS1_HUMAN
Immunogen Region: 207-256 aa
Specificity: PRAS40 Polyclonal Antibody detects endogenous levels of PRAS40 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Akt1 S1 at the amino acid range 207-256
Post Translational Modifications Phosphorylated by AKT1.phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity. Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation. Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1.
Function Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site. In response to insulin and nutrients, AKT1S1 dissociates from mTORC1. Its activity is dependent on its phosphorylation state and binding to 14-3-3. May also play a role in nerve growth factor-mediated neuroprotection.
Protein Name Proline-Rich Akt1 Substrate 1
40 Kda Proline-Rich Akt Substrate
Database Links Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-198323
Reactome: R-HSA-3371571
Reactome: R-HSA-5674400
Cellular Localisation Cytoplasm
Cytosol
Found In The Cytosolic Fraction Of The Brain
Alternative Antibody Names Anti-Proline-Rich Akt1 Substrate 1 antibody
Anti-40 Kda Proline-Rich Akt Substrate antibody
Anti-AKT1S1 antibody
Anti-PRAS40 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance