• Immunoprecipitation analysis of 25 Mu g extracts of Rat brain cells using 3 Mu g Pan-Akt antibody (STJ11100091). Western blot was performed from the immunoprecipitate using Pan-Akt antibody (STJ11100091) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using Pan-Akt Rabbit polyclonal antibody (STJ11100091) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using Pan-Akt Rabbit polyclonal antibody (STJ11100091) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Pan-Akt Rabbit polyclonal antibody (STJ11100091) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Pan-Akt in paraffin-embedded rat kidney using Pan-Akt Rabbit polyclonal antibody (STJ11100091) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Pan-Akt in paraffin-embedded rat ovary using Pan-Akt Rabbit polyclonal antibody (STJ11100091) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Pan-Akt Rabbit polyclonal antibody (STJ11100091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AKT1/AKT2/AKT3 antibody (311-480) (STJ11100091)

SKU:
STJ11100091

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pan-AKT (311-480) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 311-480
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 311-480 of human AKT1/AKT2/AKT3 (NP_005154.2).
Immunogen Sequence: GTPEYLAPEVLEDNDYGRAV DWWGLGVVMYEMMCGRLPFY NQDHEKLFELILMEEIRFPR TLGPEAKSLLSGLLKKDPKQ RLGGGSEDAKEIMQHRFFAG IVWQHVYEKKLSPPFKPQVT SETDTRYFDEEFTAQMITIT PPDQDDSMECVDSERRPHFP QFSYSASGTA
Background The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.

Information sourced from Uniprot.org

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