Anti-AKR1A1 antibody (1-325) (STJ22566)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AKR1A1 (1-325) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AKR1A1 |
| Gene ID | 10327 |
| Uniprot ID | AK1A1_HUMAN |
| Immunogen Region | 1-325 |
| Immunogen Sequence | MAASCVLLHTGQKMPLIGLG TWKSEPGQVKAAVKYALSVG YRHIDCAAIYGNEPEIGEAL KEDVGPGKAVPREELFVTSK LWNTKHHPEDVEPALRKTLA DLQLEYLDLYLMHWPYAFER GDNPFPKNADGTICYDSTHY KETWKALEALVAKGLVQALG LSNFNSRQIDDILSVASVRP AVLQVECHPYLAQNELIAHC QARGLEVTAYSPLGSSDRAW RDPDEPVLLEEPVVLALAE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human AKR1A1 (NP_006057.1). |
Additional Info
| Tissue Specificity | Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung. |
| Function | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde. Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Also acts as an inhibitor of protein S-nitrosylation by mediating degradation of S-nitroso-coenzyme A (S-nitroso-CoA), a cofactor required to S-nitrosylate proteins. S-nitroso-CoA reductase activity is involved in reprogramming intermediary metabolism in renal proximal tubules, notably by inhibiting protein S-nitrosylation of isoform 2 of PKM (PKM2). Also acts as a S-nitroso-glutathione reductase by catalyzing the NADPH-dependent reduction of S-nitrosoglutathione. Displays no reductase activity towards retinoids. |
| Protein Name | Aldo-Keto Reductase Family 1 Member A1 Alcohol Dehydrogenase Nadp(+Aldehyde ReductaseGlucuronate ReductaseGlucuronolactone ReductaseS-Nitroso-Coa ReductaseScorr |
| Database Links | Reactome: R-HSA-156590Reactome: R-HSA-5661270 |
| Cellular Localisation | CytoplasmCytosolApical Cell Membrane |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member A1 antibodyAnti-Alcohol Dehydrogenase Nadp(+ antibodyAnti-Aldehyde Reductase antibodyAnti-Glucuronate Reductase antibodyAnti-Glucuronolactone Reductase antibodyAnti-S-Nitroso-Coa Reductase antibodyAnti-Scorr antibodyAnti-AKR1A1 antibodyAnti-ALDR1 antibodyAnti-ALR antibody |
Information sourced from Uniprot.org