• Western blot analysis of extracts of various cell lines, using AK8 antibody (STJ115709) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-AK8 antibody (1-200) (STJ115709)

SKU:
STJ115709

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AK8
Gene ID: 158067
Uniprot ID: KAD8_HUMAN
Immunogen Region: 1-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human AK8 (NP_689785.1).
Immunogen Sequence: MDATIAPHRIPPEMPQYGEE NHIFELMQNMLEQLLIHQPE DPIPFMIQHLHRDNDNVPRI VILGPPASGKTTIAMWLCKH LNSSLLTLENLILNEFSYTA TEARRLYLQRKTVPSALLVQ LIQERLAEEDCIKQGWILDG IPETREQALRIQTLGITPRH VIVLSAPDTVLIERNLGKRI DPQTGEIYHTTFDWPPESEI
Tissue Specificity Expressed in respiratory cells (at protein level).
Function Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity.
Protein Name Adenylate Kinase 8
Ak 8
Atp-Amp Transphosphorylase 8
Database Links Reactome: R-HSA-499943
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Cilium Axoneme
Located In The Proximal Region Of Respiratory Cilia
Alternative Antibody Names Anti-Adenylate Kinase 8 antibody
Anti-Ak 8 antibody
Anti-Atp-Amp Transphosphorylase 8 antibody
Anti-AK8 antibody
Anti-C9orf98 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance