Anti-AJUBA antibody (STJA0008669)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human Ajuba
General Information
| Short Description | Rabbit polyclonal anti-LIM domain-containing protein ajuba for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AJUBA |
| Gene ID | 84962 |
| Uniprot ID | AJUBA_HUMAN |
| Immunogen | Synthesized peptide derived from human Ajuba |
| Specificity | This antibody detects endogenous levels of Ajuba at Human, Mouse, Rat |
Additional Info
| Function | Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. |
| Protein Name | Lim Domain-Containing Protein Ajuba |
| Database Links | Reactome: R-HSA-1234176Reactome: R-HSA-2565942Reactome: R-HSA-5617472Reactome: R-HSA-9841922 |
| Cellular Localisation | CytoplasmCytoskeletonCell MembraneCell JunctionNucleusMicrotubule Organizing CenterCentrosomeP-BodyShuttles Between The Cytoplasm And The NucleusLocalizes On Centrosomes During G2-M PhasePreferentially Co- Localizes With Cadherin-Adhesive Complexes At Sites Of Cell-Cell ContactsColocalizes With Gfi1 In The Nucleus |
| Alternative Antibody Names | Anti-Lim Domain-Containing Protein Ajuba antibodyAnti-AJUBA antibodyAnti-JUB antibody |
Information sourced from Uniprot.org