| Tissue Specificity | Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells. |
| Post Translational Modifications | N-myristoylation at Gly-2 mediates the recruitment to lipid droplets and plasma membrane, enabling its anti-lipid peroxidation activity. Acetylation at Lys-168 prevents AIFM2 ubiquitination and degradation, thereby inhibiting ferroptosis. KAT2B mediates acetylation at Lys-168, while HDAC3 removes it. Ubiquitinated. AIFM2 undergoes 'Lys-29'-ubiquitination and proteasomal degradation, which is inhibited by acetylation at Lys-168. |
| Function | A NAD(P)H-dependent oxidoreductase that acts as a key inhibitor of ferroptosis. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Acts in parallel to GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels. Also acts as a potent radical-trapping antioxidant by mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle: catalyzes NAD(P)H-dependent reduction of vitamin K (phylloquinone, menaquinone-4 and menadione) to hydroquinone forms. Hydroquinones act as potent radical-trapping antioxidants inhibitor of phospholipid peroxidation and ferroptosis. May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death. Capable of DNA binding in a non-sequence specific way. |
| Protein Name | Ferroptosis Suppressor Protein 1Fsp1Apoptosis-Inducing Factor Homologous Mitochondrion-Associated Inducer Of DeathAmidP53-Responsive Gene 3 Protein |
| Database Links | Reactome: R-HSA-6803204 |
| Cellular Localisation | Lipid DropletCell MembraneLipid-AnchorCytoplasmMitochondrion MembraneNucleus |
| Alternative Antibody Names | Anti-Ferroptosis Suppressor Protein 1 antibodyAnti-Fsp1 antibodyAnti-Apoptosis-Inducing Factor Homologous Mitochondrion-Associated Inducer Of Death antibodyAnti-Amid antibodyAnti-P53-Responsive Gene 3 Protein antibodyAnti-AIFM2 antibodyAnti-AMID antibodyAnti-PRG3 antibody |
Information sourced from Uniprot.org
