| Host: | Goat |
| Applications: | Pep-ELISA/FC/IF/IHC |
| Reactivity: | Human/Mouse/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-AIF1/IBA1-Isoform 3 (Internal) is suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.IHC: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6µg/ml.IF: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | AIF1 |
| Gene ID: | 199 |
| Uniprot ID: | AIF1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001614.3 |
| Specificity: | This antibody is expected to recognise isoform 3 (NP_001614.3) only. |
| Immunogen Sequence: | NKQFLDDPKYSSDED |
| Post Translational Modifications | Phosphorylated on serine residues. |
| Function | Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. |
| Protein Name | Allograft Inflammatory Factor 1Aif-1Ionized Calcium-Binding Adapter Molecule 1Protein G1 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SidePhagocytic CupAssociated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis |
| Alternative Antibody Names | Anti-Allograft Inflammatory Factor 1 antibodyAnti-Aif-1 antibodyAnti-Ionized Calcium-Binding Adapter Molecule 1 antibodyAnti-Protein G1 antibodyAnti-AIF1 antibodyAnti-G1 antibodyAnti-IBA1 antibody |
Information sourced from Uniprot.org