| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-AIF1/IBA1 isoform 1 and 3 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 18kDa band observed in Human Frontal Cortex lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
AIF1 |
| Gene ID: |
199 |
| Uniprot ID: |
AIF1_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_116573.1; NP_001614.3 |
| Immunogen Sequence: |
TGPPAKKAISELP.. |
| Post Translational Modifications | Phosphorylated on serine residues. |
| Function | Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. |
| Protein Name | Allograft Inflammatory Factor 1Aif-1Ionized Calcium-Binding Adapter Molecule 1Protein G1 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SidePhagocytic CupAssociated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis |
| Alternative Antibody Names | Anti-Allograft Inflammatory Factor 1 antibodyAnti-Aif-1 antibodyAnti-Ionized Calcium-Binding Adapter Molecule 1 antibodyAnti-Protein G1 antibodyAnti-AIF1 antibodyAnti-G1 antibodyAnti-IBA1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
