• Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-AFP (PT0679) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HepG2 Lane 3: mouse liverPredicted band size: 69kDaObserved band size: 69kDa

Anti-AFP antibody (350-450 aa) (STJ196165)

SKU:
STJ196165

Current Stock:
Host: Mouse
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Alpha-fetoprotein (350-450 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:500-2000
IHC 1:100-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AFP
Gene ID: 174
Uniprot ID: FETA_HUMAN
Immunogen Region: 350-450 aa
Specificity: This antibody detects endogenous levels of human Alpha-1-Fetoprotein (AFP)
Immunogen: Synthesized peptide derived from the human Alpha-1-Fetoprotein (AFP) at the amino acid range 350-450
Post Translational Modifications Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated.
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Protein Name Alpha-Fetoprotein
Alpha-1-Fetoprotein
Alpha-Fetoglobulin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-Fetoprotein antibody
Anti-Alpha-1-Fetoprotein antibody
Anti-Alpha-Fetoglobulin antibody
Anti-AFP antibody
Anti-HPAFP antibody

Information sourced from Uniprot.org

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