![Western blot analysis of lysates from wild type (WT) and AES knockout (KO) HeLa cells, using [KO Validated] AES Rabbit polyclonal antibody (STJ11100820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89756/358232/STJ11100820_1__13212.1713122563.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AES knockout (KO) HeLa cells, using [KO Validated] AES Rabbit polyclonal antibody (STJ11100820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and AES knockout (KO) HeLa cells, using [KO Validated] AES Rabbit polyclonal antibody (STJ11100820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89756/358232/STJ11100820_1__13212.1713122563.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AES knockout (KO) HeLa cells, using [KO Validated] AES Rabbit polyclonal antibody (STJ11100820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-AES antibody (1-264) (STJ11100820)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AES (1-264) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLE5 |
| Gene ID | 166 |
| Uniprot ID | TLE5_HUMAN |
| Immunogen Region | 1-264 |
| Immunogen Sequence | MCHKNGFPQEGGITAAFLQK RKLRLSKNHRPARAKVTEHV RGTRPGRATAGPAASTRAAG SLFFDRWGNRGPAGCRGSSH LPQQLKFTTSDSCDRIKDEF QLLQAQYHSLKLECDKLASE KSEMQRHYVMYYEMSYGLNI EMHKQAEIVKRLNGICAQVL PYLSQEHQQQVLGAIERAKQ VTAPELNSIIRQQLQAHQLS QLQALALPLTPLPVGLQPPS LPAVSAGTGLLSLSALGSQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1). |
Additional Info
| Tissue Specificity | Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver. |
| Post Translational Modifications | Ubiquitinated by XIAP/BIRC4. |
| Function | Transcriptional corepressor. Acts as a dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. |
| Protein Name | Tle Family Member 5Amino-Terminal Enhancer Of SplitAmino Enhancer Of SplitGp130-Associated Protein GamGrg-5Groucho-Related Protein 5Protein Esp1Protein GrgTle Family Member 5 - Transcriptional Modulator |
| Database Links | Reactome: R-HSA-4641265 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Tle Family Member 5 antibodyAnti-Amino-Terminal Enhancer Of Split antibodyAnti-Amino Enhancer Of Split antibodyAnti-Gp130-Associated Protein Gam antibodyAnti-Grg-5 antibodyAnti-Groucho-Related Protein 5 antibodyAnti-Protein Esp1 antibodyAnti-Protein Grg antibodyAnti-Tle Family Member 5 - Transcriptional Modulator antibodyAnti-TLE5 antibodyAnti-AES antibodyAnti-GRG antibodyAnti-GRG5 antibody |
Information sourced from Uniprot.org