• Western blot analysis of extracts of various cell lines, using ADRB3 antibody (STJ111338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of C6 cells using ADRB3 Rabbit polyclonal antibody (STJ111338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADRB3 rabbit polyclonal antibody (STJ111338) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ADRB3 Rabbit polyclonal antibody (STJ111338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ADRB3 rabbit polyclonal antibody (STJ111338) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADRB3 Rabbit polyclonal antibody (STJ111338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ADRB3 rabbit polyclonal antibody (STJ111338) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ADRB3 Rabbit polyclonal antibody (STJ111338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADRB3 antibody (1-100) (STJ111338)

SKU:
STJ111338

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADRB3 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADRB3
Gene ID: 155
Uniprot ID: ADRB3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ADRB3 (NP_000016.1).
Immunogen Sequence: MAPWPHENSSLAPWPDLPTL APNTANTSGLPGVPWEAALA GALLALAVLATVGGNLLVIV AIAWTPRLQTMTNVFVTSLA AADLVMGLLVVPPAATLALT
Tissue Specificity Expressed mainly in adipose tissues.
Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Protein Name Beta-3 Adrenergic Receptor
Beta-3 Adrenoreceptor
Beta-3 Adrenoceptor
Database Links Reactome: R-HSA-390696
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Beta-3 Adrenergic Receptor antibody
Anti-Beta-3 Adrenoreceptor antibody
Anti-Beta-3 Adrenoceptor antibody
Anti-ADRB3 antibody
Anti-ADRB3R antibody
Anti-B3AR antibody

Information sourced from Uniprot.org

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