STJ70868 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ADRA2A antibody (Internal) (STJ70868)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70868-100
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General Information

Short DescriptionGoat polyclonal antibody anti-ADRA2A (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Mouse/Rat/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
IF: Strong expression of the protein seen in the membranes of MCF7 cells. Recommended concentration: 10ยตg/ml.

FC:Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/m

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolADRA2A
Gene ID150
Uniprot IDADA2A_HUMAN
Accession NumberNP_000672.3
Immunogen RegionInternal
Immunogen SequenceTERRPNGLGPERS

Additional Info

Protein Name Alpha-2a Adrenergic Receptor
Alpha-2 Adrenergic Receptor Subtype C10
Alpha-2a Adrenoreceptor
Alpha-2a Adrenoceptor
Alpha-2aar
Database Links Reactome: R-HSA-390696
Reactome: R-HSA-392023
Reactome: R-HSA-400042
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Reactome: R-HSA-5683826
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Alpha-2a Adrenergic Receptor antibody
Anti-Alpha-2 Adrenergic Receptor Subtype C10 antibody
Anti-Alpha-2a Adrenoreceptor antibody
Anti-Alpha-2a Adrenoceptor antibody
Anti-Alpha-2aar antibody
Anti-ADRA2A antibody
Anti-ADRA2R antibody
Anti-ADRAR antibody

Information sourced from Uniprot.org

Citations

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