• Western blot analysis of the lysates from HeLa cells using GPR116 antibody.
  • Immunofluorescence analysis of HeLa cells, using GPR116 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from A549 cells, using GPR116 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using GPR116 Polyclonal Antibody diluted at 1：500

Anti-ADGRF5 antibody (11-60 aa) (STJ93325)

SKU:
STJ93325

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Adhesion G protein-coupled receptor F5 (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADGRF5
Gene ID: 221395
Uniprot ID: AGRF5_HUMAN
Immunogen Region: 11-60 aa
Specificity: GPR116 Polyclonal Antibody detects endogenous levels of GPR116 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GPR116 at the amino acid range 11-60
Post Translational Modifications Highly glycosylated. Proteolytically cleaved at multiple sites: one in the GPS region of the GAIN-B domain (S1 site) and the other in the SEA domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. The proteolytic cleavage at S2 site generates a fragment that undergoes proteolytic cleavage by the processing enzyme furin.
Function Adhesion G protein-coupled receptor. In alveolar type II (ATII or AT2) cells, required for normal lung surfactant homeostasis. Modulation of both surfactant secretion and uptake by ATII cells is mediated by the downstream activation of GNAQ/GNA11 proteins and may be a consequence of increased cortical F-actin assembly induced by ADGRF5 activation. In the kidney, may play a role in the regulation of acid excretion into the primary urine, possibly by regulating the surface expression of V-ATPase proton pump. As a receptor for soluble FNDC4 (sFNDC4), required for proper systemic glucose tolerance, specifically sensitizing white adipose tissue to insulin. Plays also a role in sFNDC4-induced decrease of local inflammation in white adipose tissue.
Protein Name Adhesion G Protein-Coupled Receptor F5
G-Protein Coupled Receptor 116
Database Links Reactome: R-HSA-5683826
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Adhesion G Protein-Coupled Receptor F5 antibody
Anti-G-Protein Coupled Receptor 116 antibody
Anti-ADGRF5 antibody
Anti-GPR116 antibody
Anti-KIAA0758 antibody

Information sourced from Uniprot.org

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