Anti-ADAM15 antibody (200-300) [S5MR] (STJ11102325)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ADAM15 (200-300) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:200-1:2000IF/ICC:1:200-1:800ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ADAM15 |
| Gene ID | 8751 |
| Uniprot ID | ADA15_HUMAN |
| Immunogen Region | 200-300 |
| Immunogen Sequence | RHIRRRRDVVTETKTVELVI VADHSEAQKYRDFQHLLNRT LEVALLLDTFFRPLNVRVAL VGLEAWTQRDLVEISPNPAV TLENFLHWRRAHLLPRLPHD S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ADAM15 (Q13444). |
Additional Info
| Tissue Specificity | Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells. |
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. Phosphorylation increases association with PTKs. |
| Function | Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. |
| Protein Name | Disintegrin And Metalloproteinase Domain-Containing Protein 15Adam 15Metalloprotease Rgd Disintegrin ProteinMetalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein 15Mdc-15Metargidin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-8941237 |
| Cellular Localisation | Endomembrane SystemSingle-Pass Type I Membrane ProteinCell JunctionAdherens JunctionCell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeThe Majority Of The Protein Is Localized In A Perinuclear Compartment Which May Correspond To The Trans-Golgi Network Or The Late EndosomeThe Pro-Protein Is The Major Detectable Form On The Cell SurfaceWhereas The Majority Of The Protein In The Cell Is Processed |
| Alternative Antibody Names | Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 15 antibodyAnti-Adam 15 antibodyAnti-Metalloprotease Rgd Disintegrin Protein antibodyAnti-Metalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein 15 antibodyAnti-Mdc-15 antibodyAnti-Metargidin antibodyAnti-ADAM15 antibodyAnti-MDC15 antibody |
Information sourced from Uniprot.org