• STJ70610 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic/plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70610 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0. 4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ70610 (3. 8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-ADAM12 antibody (N-Term) (STJ70610)

SKU:
STJ70610-100

£303.50
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Host: Goat
Applications: Pep-ELISA/FC/IF/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ADAM12 (N-Term) is suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3-5µg/ml, 3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm and plasma membranes of A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ADAM12
Gene ID: 8038
Uniprot ID: ADA12_HUMAN
Immunogen Region: N-Term
Accession Number: NP_003465.3; NP_067673.2
Specificity: This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
Immunogen Sequence: AARPLPVSPARALC
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Protein Name Disintegrin And Metalloproteinase Domain-Containing Protein 12
Adam 12
Meltrin-Alpha
Database Links Reactome: R-HSA-177929
Reactome: R-HSA-8941237
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Alternative Antibody Names Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 12 antibody
Anti-Adam 12 antibody
Anti-Meltrin-Alpha antibody
Anti-ADAM12 antibody
Anti-MLTN antibody
Anti-UNQ346 antibody
Anti-PRO545 antibody

Information sourced from Uniprot.org

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