• Western blot analysis of extracts of various cell lines, using ADAM12 antibody (STJ110249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of NIH/3T3 cells using ADAM12 antibody (STJ110249) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat heart using ADAM12 antibody (STJ110249) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of HeLa cells using ADAM12 antibody (STJ110249) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ADAM12 antibody (STJ110249) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ADAM12 antibody (STJ110249) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ADAM12 antibody (STJ110249) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using ADAM12 Rabbit polyclonal antibody (STJ110249) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using ADAM12 antibody (STJ110249) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ADAM12 antibody (STJ110249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ADAM12 antibody (589-738) (STJ110249)

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STJ110249

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAM12 (589-738) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAM12
Gene ID: 8038
Uniprot ID: ADA12_HUMAN
Immunogen Region: 589-738
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 589-738 of human ADAM12 (NP_067673.2).
Immunogen Sequence: ASRPVIGTNAVSIETNIPLQ QGGRILCRGTHVYLGDDMPD PGLVLAGTKCADGKICLNRQ CQNISVFGVHECAMQCHGRG VCNNRKNCHCEAHWAPPFCD KFGFGGSTDSGPIRQAEARQ EAAESNRERGQGQEPVGSQE HASTASLTLI
Tissue Specificity Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Protein Name Disintegrin And Metalloproteinase Domain-Containing Protein 12
Adam 12
Meltrin-Alpha
Database Links Reactome: R-HSA-177929
Reactome: R-HSA-8941237
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Alternative Antibody Names Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 12 antibody
Anti-Adam 12 antibody
Anti-Meltrin-Alpha antibody
Anti-ADAM12 antibody
Anti-MLTN antibody
Anti-UNQ346 antibody
Anti-PRO545 antibody

Information sourced from Uniprot.org

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