| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ADA (1-363) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ADA |
| Gene ID: |
100 |
| Uniprot ID: |
ADA_HUMAN |
| Immunogen Region: |
1-363 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Adenosine Deaminase (Adenosine Deaminase (ADA) ) (NP_000013.2). |
| Immunogen Sequence: |
MAQTPAFDKPKVELHVHLDG SIKPETILYYGRRRGIALPA NTAEGLLNVIGMDKPLTLPD FLAKFDYYMPAIAGCREAIK RIAYEFVEMKAKEGVVYVEV RYSPHLLANSKVEPIPWNQA EGDLTPDEVVALVGQGLQEG ERDFGVKARSILCCMRHQPN WSPKVVELCKKYQQQTVVAI DLAGDETIPGSSLLPGHVQA YQEAVKSGIHRTVHAGEVGS AEVVKEAVDILKTERLGHG |
| Tissue Specificity | Found in all tissues, occurs in large amounts in T-lymphocytes. Expressed at the time of weaning in gastrointestinal tissues. |
| Function | Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development. |
| Protein Name | Adenosine DeaminaseAdenosine Aminohydrolase |
| Database Links | Reactome: R-HSA-74217Reactome: R-HSA-9734735Reactome: R-HSA-9755088 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinExtracellular SideCell JunctionCytoplasmic Vesicle LumenCytoplasmLysosomeColocalized With Dpp4 At The Cell Surface |
| Alternative Antibody Names | Anti-Adenosine Deaminase antibodyAnti-Adenosine Aminohydrolase antibodyAnti-ADA antibodyAnti-ADA1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA) ) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit polyclonal antibody (STJ11100002) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89075/357532/STJ11100002_1__24167.1713121647.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA) ) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit polyclonal antibody (STJ11100002) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
