• STJ71283 (1µg/ml) staining of Human Brain (Substantia nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Acylglycerol kinase antibody (C-Term) (STJ71283)

SKU:
STJ71283-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Acylglycerol kinase (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AGK
Gene ID: 55750
Uniprot ID: AGK_HUMAN
Immunogen Region: C-Term
Accession Number: NP_060708.1
Immunogen Sequence: CDPRKREQMLTSP
Function Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Phosphorylates ceramide. Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol. Independently of its lipid kinase activity, acts as a component of the TIM22 complex. The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force. In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
Protein Name Acylglycerol Kinase - Mitochondrial
Hagk
Multiple Substrate Lipid Kinase
Hsmulk
Mulk
Multi-Substrate Lipid Kinase
Database Links Reactome: R-HSA-1483206
Reactome: R-HSA-6802952
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Mitochondrion Intermembrane Space
Localizes In The Mitochondrion Intermembrane Space
Where It Associates With The Inner Membrane
It Is Unclear Whether The N-Terminal Hydrophobic Region Forms A Transmembrane Region Or Associates With The Membrane Without Crossing It
Alternative Antibody Names Anti-Acylglycerol Kinase - Mitochondrial antibody
Anti-Hagk antibody
Anti-Multiple Substrate Lipid Kinase antibody
Anti-Hsmulk antibody
Anti-Mulk antibody
Anti-Multi-Substrate Lipid Kinase antibody
Anti-AGK antibody
Anti-MULK antibody

Information sourced from Uniprot.org

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