• Immunofluorescence analysis of MCF7 cells using ACY1 antibody (STJ115419). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using ACY1 antibody (STJ115419) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ACY1 antibody (STJ115419) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ACY1 antibody (STJ115419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ACY1 antibody (1-408) (STJ115419)

SKU:
STJ115419

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACY1 (1-408) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACY1
Gene ID: 95
Uniprot ID: ACY1_HUMAN
Immunogen Region: 1-408
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-408 of human ACY1 (NP_001185824.1).
Immunogen Sequence: MTSKGPEEEHPSVTLFRQYL RIRTVQPKPDYGAAVAFFEE TARQLGLGCQKVEVAPGYVV TVLTWPGTNPTLSSILLNSH TDVVPVFKEHWSHDPFEAFK DSEGYIYARGAQDMKCVSIQ YLEAVRRLKVEGHRFPRTIH MTFVPDEEVGGHQGMELFVQ RPEFHALRAGFALDEGIANP TDAFTVFYSERSPWWVRVTS TGRPGHASRFMEDTAAEKLH KVVNSILAFREKEWQRLQS
Tissue Specificity Expression is highest in kidney, strong in brain and weaker in placenta and spleen.
Function Catalyzes the hydrolysis of N-acetylated amino acids to acetate and free amino acids.
Protein Name Aminoacylase-1
Acy-1
N-Acyl-L-Amino-Acid Amidohydrolase
Database Links Reactome: R-HSA-5423646
Reactome: R-HSA-5579007
Reactome: R-HSA-9753281
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Aminoacylase-1 antibody
Anti-Acy-1 antibody
Anti-N-Acyl-L-Amino-Acid Amidohydrolase antibody
Anti-ACY1 antibody

Information sourced from Uniprot.org

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