• Immunofluorescence analysis of U2OS cells using Arp2 antibody (STJ28301). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Arp2 antibody (STJ28301) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human uterine cancer using Arp2 antibody (STJ28301) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Arp2 antibody (STJ28301) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Arp2 antibody (STJ28301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACTR2 antibody (1-160) (STJ28301)

SKU:
STJ28301

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACTR2 (1-160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTR2
Gene ID: 10097
Uniprot ID: ARP2_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human Arp2 (NP_005713.1).
Immunogen Sequence: MDSQGRKVVVCDNGTGFVKC GYAGSNFPEHIFPALVGRPI IRSTTKVGNIEIKDLMVGDE ASELRSMLEVNYPMENGIVR NWDDMKHLWDYTFGPEKLNI DTRNCKILLTEPPMNPTKNR EKIVEVMFETYQFSGVYVAI QAVLTLYAQGLLTGVVVDSG
Function ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Protein Name Actin-Related Protein 2
Actin-Like Protein 2
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-5663213
Reactome: R-HSA-6798695
Reactome: R-HSA-8856828
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Nucleus
Alternative Antibody Names Anti-Actin-Related Protein 2 antibody
Anti-Actin-Like Protein 2 antibody
Anti-ACTR2 antibody
Anti-ARP2 antibody

Information sourced from Uniprot.org

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