• Immunohistochemistry analysis of paraffin-embedded mouse heart using ACTN1 antibody (STJ22500) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using ACTN1 antibody (STJ22500) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using ACTN1 antibody (STJ22500) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of NIH/3T3, using ACTN1 antibody (STJ22500) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ACTN1 antibody (1-330) (STJ22500)

SKU:
STJ22500

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACTN1 (1-330) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN1
Gene ID: 87
Uniprot ID: ACTN1_HUMAN
Immunogen Region: 1-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1).
Immunogen Sequence: MDHYDSQQTNDYMQPEEDWD RDLLLDPAWEKQQRKTFTAW CNSHLRKAGTQIENIEEDFR DGLKLMLLLEVISGERLAKP ERGKMRVHKISNVNKALDFI ASKGVKLVSIGAEEIVDGNV KMTLGMIWTIILRFAIQDIS VEETSAKEGLLLWCQRKTAP YKNVNIQNFHISWKDGLGFC ALIHRHRPELIDYGKLRKDD PLTNLNTAFDVAEKYLDIPK MLDAEDIVGTARPDEKAIM
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name Alpha-Actinin-1
Alpha-Actinin Cytoskeletal Isoform
F-Actin Cross-Linking Protein
Non-Muscle Alpha-Actinin-1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-3000170
Reactome: R-HSA-373753
Reactome: R-HSA-446388
Reactome: R-HSA-9013405
Reactome: R-HSA-9013418
Reactome: R-HSA-9035034
Cellular Localisation Cytoplasm
Cytoskeleton
Myofibril
Sarcomere
Z Line
Cell Membrane
Cell Junction
Cell Projection
Ruffle
Colocalizes With Myoz2 And Ppp3ca At The Z-Line Of Heart And Skeletal Muscle
Colocalizes With Psd In Membrane Ruffles And Central Reticular Structures
Alternative Antibody Names Anti-Alpha-Actinin-1 antibody
Anti-Alpha-Actinin Cytoskeletal Isoform antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-Non-Muscle Alpha-Actinin-1 antibody
Anti-ACTN1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance