Western blot analysis of lysates from 293, HeLa, and A549 cells, using ACTN1/2/3/4 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Actinin-Alpha 1/2/3/4 antibody (21-70 aa) (STJ91466)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ACTN1/2/3/4 at the amino acid range 21-70
STJ91466
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General Information

Short DescriptionRabbit polyclonal anti-Alpha-actinin-1 and Alpha-actinin-2 and Alpha-actinin-3 and Alpha-actinin-4 (21-70 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolACTN1
ACTN4
87
81
89
ACTN1_HUMAN
ACTN4_HUMAN
ACTN3_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ACTN1/2/3/4 at the amino acid range 21-70
Immunogen Region21-70 aa
SpecificityActinin-Alpha 1/2/3/4 Polyclonal Antibody detects endogenous levels of Actinin-Alpha 1/2/3/4 protein.

Additional Info

Protein Name Alpha-Actinin-1
Alpha-Actinin Cytoskeletal Isoform
F-Actin Cross-Linking Protein
Non-Muscle Alpha-Actinin-1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-3000170
Reactome: R-HSA-373753
Reactome: R-HSA-446388
Cellular Localisation Cytoplasm
Cytoskeleton
Myofibril
Sarcomere
Z Line
Cell Membrane
Cell Junction
Cell Projection
Ruffle
Colocalizes With Myoz2 And Ppp3ca At The Z-Line Of Heart And Skeletal Muscle
Colocalizes With Psd In Membrane Ruffles And Central Reticular Structures
Alternative Antibody Names Anti-Alpha-Actinin-1 antibody
Anti-Alpha-Actinin Cytoskeletal Isoform antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-Non-Muscle Alpha-Actinin-1 antibody
Anti-ACTN1 antibody

Information sourced from Uniprot.org

Citations

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