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Anti-Actin antibody (326-375 aa) (STJ91461)

Western blot analysis of lysates from HeLa cells, using Actin-pan Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actin-pan Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000
Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000
Western blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1：2000
Western blot analysis of various cells using Actin Polyclonal Antibody diluted at 1：2000

Anti-Actin antibody (326-375 aa) (STJ91461)

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STJ91461

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat/Fish
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Actin, cytoplasmic 1 and Putative beta-actin-like protein 3 and Actin, cytoplasmic 2 (326-375 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	ACTB ACTG1 60 71 NA ACTB_HUMAN ACTG_HUMAN ACTBM_HUMAN
Immunogen Region:	326-375 aa
Specificity:	Actin Polyclonal Antibody detects endogenous levels of Actin protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Actin at the amino acid range 326-375

Function	Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Protein Name	Actin - Cytoplasmic 1 Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed
Database Links	Reactome: R-HSA-1445148 Reactome: R-HSA-190873 Reactome: R-HSA-196025 Reactome: R-HSA-2029482 Reactome: R-HSA-3214847 Reactome: R-HSA-389957 Reactome: R-HSA-390450 Reactome: R-HSA-3928662 Reactome: R-HSA-3928665 Reactome: R-HSA-418990 Reactome: R-HSA-437239 Reactome: R-HSA-4420097 Reactome: R-HSA-445095 Reactome: R-HSA-446353 Reactome: R-HSA-5250924 Reactome: R-HSA-5626467 Reactome: R-HSA-5663213 Reactome: R-HSA-5663220 Reactome: R-HSA-5674135 Reactome: R-HSA-5689603 Reactome: R-HSA-5696394 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-8856828 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9664422 Reactome: R-HSA-983231
Cellular Localisation	Cytoplasm Cytoskeleton Nucleus Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names	Anti-Actin - Cytoplasmic 1 antibody Anti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibody Anti-ACTB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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