• Western blot analysis of extracts of various cell lines, using Beta-actin antibody (STJ113532) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of Beta-Actin in paraffin-embedded Rat testis tissue using Beta-Actin Rabbit monoclonal antibody (High Dilution) (STJ113532) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of extracts of HeLa cells, using Beta-actin antibody (STJ113532) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Beta-Actin in paraffin-embedded Human liver cancer tissue using Beta-Actin Rabbit monoclonal antibody (High Dilution) (STJ113532) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Beta-Actin in paraffin-embedded Mouse intestin tissue using Beta-Actin Rabbit monoclonal antibody (High Dilution) (STJ113532) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from HeLa cells, using Beta-Actin Rabbit monoclonal antibody (High Dilution) (STJ113532) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using Beta-Actin Rabbit monoclonal antibody (High Dilution) (STJ113532) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACTB antibody [S2MR] (STJ113532)

SKU:
STJ113532

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat/Chicken/Zebrafish/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ACTB is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:50000-1:200000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTB
Gene ID: 60
Uniprot ID: ACTB_HUMAN
Immunogen: Recombinant protein of human Beta-actin
Immunogen Sequence: MDDDIAALVVDNGSGMCKAG FAGDDAPRAVFPSIVGRPRH QGVMVGMGQKDSYVGDEAQS KRGILTLKYPIEHGIVTNWD DMEKIWHHTFYNELRVAPEE HPVLLTEAPLNPKANREKMT QIMFETFNTPAMYVAIQAVL SLYASGRTTGIVMDSGDGVT HTVPIYEGYALPHAILRLDL AGRDLTDYLMKILTERGYSF TTTAEREIVRDIKEKLCYVA LDFEQEMATAASSSSLEKS
Post Translational Modifications ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery. Actin, cytoplasmic 1: N-terminal cleavage of acetylated methionine of immature cytoplasmic actin by ACTMAP. Actin, cytoplasmic 1, N-terminally processed: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules.
Protein Name Actin - Cytoplasmic 1
Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-190873
Reactome: R-HSA-196025
Reactome: R-HSA-2029482
Reactome: R-HSA-3214847
Reactome: R-HSA-389957
Reactome: R-HSA-390450
Reactome: R-HSA-3928662
Reactome: R-HSA-3928665
Reactome: R-HSA-418990
Reactome: R-HSA-437239
Reactome: R-HSA-4420097
Reactome: R-HSA-445095
Reactome: R-HSA-446353
Reactome: R-HSA-5250924
Reactome: R-HSA-5626467
Reactome: R-HSA-5663213
Reactome: R-HSA-5663220
Reactome: R-HSA-5674135
Reactome: R-HSA-5689603
Reactome: R-HSA-5696394
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-8856828
Reactome: R-HSA-9035034
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Reactome: R-HSA-9664422
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Actin - Cytoplasmic 1 antibody
Anti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibody
Anti-ACTB antibody

Information sourced from Uniprot.org

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