Western blot analysis of lysates from 1) Jurkat, 2) K562, 3) HuvEC cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-ACTA2 antibody (84-134 aa) (STJ96418)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the N-terminal region of human Alpha-SMA at the amino acid range 84-134
STJ96418
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General Information

Short DescriptionRabbit polyclonal anti-Actin, aortic smooth muscle (84-134 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolACTA2
Gene ID59
Uniprot IDACTA_HUMAN
ImmunogenSynthesized peptide derived from the N-terminal region of human Alpha-SMA at the amino acid range 84-134
Immunogen Region84-134 aa
SpecificityAlpha-SMA Polyclonal Antibody detects endogenous levels of Alpha-SMA protein.

Additional Info

Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Aortic Smooth Muscle
Alpha-Actin-2
Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-9013695
Reactome: R-HSA-9913351
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Actin - Aortic Smooth Muscle antibody
Anti-Alpha-Actin-2 antibody
Anti-Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form antibody
Anti-ACTA2 antibody
Anti-ACTSA antibody
Anti-ACTVS antibody
Anti-GIG46 antibody

Information sourced from Uniprot.org

Citations

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