• Western blot analysis of various lysates, using ACOX2 antibody (STJ114662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of mouse kidney, using ACOX2 antibody (STJ114662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using ACOX2 antibody (STJ114662) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ACOX2 antibody (1-150) (STJ114662)

SKU:
STJ114662

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACOX2 (1-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACOX2
Gene ID: 8309
Uniprot ID: ACOX2_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human ACOX2 (NP_003491.1).
Immunogen Sequence: MGSPVHRVSLGDTWSRQMHP DIESERYMQSFDVERLTNIL DGGAQNTALRRKVESIIHSY PEFSCKDNYFMTQNERYKAA MRRAFHIRLIARRLGWLEDG RELGYAYRALSGDVALNIHR VFVRALRSLGSEEQIAKWDP LCKNIQIIAT
Tissue Specificity Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.
Function Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids. Capable of oxidizing short as well as long chain 2-methyl branched fatty acids.
Protein Name Peroxisomal Acyl-Coenzyme A Oxidase 2
3-Alpha -7-Alpha -12-Alpha-Trihydroxy-5-Beta-Cholestanoyl-Coa 24-Hydroxylase
3-Alpha -7-Alpha -12-Alpha-Trihydroxy-5-Beta-Cholestanoyl-Coa Oxidase
Trihydroxycoprostanoyl-Coa Oxidase
Thca-Coa Oxidase
Thccox
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-389887
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Peroxisomal Acyl-Coenzyme A Oxidase 2 antibody
Anti-3-Alpha -7-Alpha -12-Alpha-Trihydroxy-5-Beta-Cholestanoyl-Coa 24-Hydroxylase antibody
Anti-3-Alpha -7-Alpha -12-Alpha-Trihydroxy-5-Beta-Cholestanoyl-Coa Oxidase antibody
Anti-Trihydroxycoprostanoyl-Coa Oxidase antibody
Anti-Thca-Coa Oxidase antibody
Anti-Thccox antibody
Anti-ACOX2 antibody

Information sourced from Uniprot.org

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