• Western blot analysis of extracts of various cell lines, using ACLY antibody (STJ117446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using ACLY antibody (STJ117446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ACLY rabbit polyclonal antibody (STJ117446) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACLY antibody (752-1101) (STJ117446)

SKU:
STJ117446

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACLY (752-1101) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACLY
Gene ID: 47
Uniprot ID: ACLY_HUMAN
Immunogen Region: 752-1101
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_001087.2).
Immunogen Sequence: FSSEVQFGHAGACANQASET AVAKNQALKEAGVFVPRSFD ELGEIIQSVYEDLVANGVIV PAQEVPPPTVPMDYSWAREL GLIRKPASFMTSICDERGQE LIYAGMPITEVFKEEMGIGG VLGLLWFQKRLPKYSCQFIE MCLMVTADHGPAVSGAHNTI ICARAGKDLVSSLTSGLLTI GDRFGGALDAAAKMFSKAFD SGIIPMEFVNKMKKEGKLIM GIGHRVKSINNPDMRVQIL
Post Translational Modifications Phosphorylated by PKA and GSK3 in a sequential manner.phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455. Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues. ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells.
Function Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.
Protein Name Atp-Citrate Synthase
Atp-Citrate
Pro-S--Lyase
Acl
Citrate Cleavage Enzyme
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-6798695
Reactome: R-HSA-75105
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Atp-Citrate Synthase antibody
Anti-Atp-Citrate antibody
Anti-Pro-S--Lyase antibody
Anti-Acl antibody
Anti-Citrate Cleavage Enzyme antibody
Anti-ACLY antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance