Anti-Acetyl-XRCC6-Lys331 antibody (291-340 aa) (STJ90158)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized Acetyl-peptide derived from the human Ku70 around the Acetylation site of Lys331 at the amino acid range 291-340
General Information
| Short Description | Rabbit polyclonal anti-Acetyl-X-ray repair cross-complementing protein 6-Lys331 (291-340 aa) for use in WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | XRCC6 |
| Gene ID | 2547 |
| Uniprot ID | XRCC6_HUMAN |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from the human Ku70 around the Acetylation site of Lys331 at the amino acid range 291-340 |
| Immunogen Region | 291-340 aa |
| Specificity | Acetyl-Ku-70 (K331) Polyclonal Antibody detects endogenous levels of Ku-70 protein only when acetylated at K331. |
Additional Info
| Function | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. Negatively regulates apoptosis by interacting with BAX and sequestering it from the mitochondria. Might have deubiquitination activity, acting on BAX. |
| Protein Name | X-Ray Repair Cross-Complementing Protein 65'-Deoxyribose-5-Phosphate Lyase Ku705'-Drp Lyase Ku7070 Kda Subunit Of Ku AntigenAtp-Dependent Dna Helicase 2 Subunit 1Atp-Dependent Dna Helicase Ii 70 Kda SubunitCtc Box-Binding Factor 75 Kda SubunitCtc75CtcbfDna Repair Protein Xrcc6Lupus Ku Autoantigen Protein P70Ku70Thyroid-Lupus AutoantigenTlaaX-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 |
| Database Links | Reactome: R-HSA-164843Reactome: R-HSA-1834949Reactome: R-HSA-3270619Reactome: R-HSA-5693571Reactome: R-HSA-6798695 |
| Cellular Localisation | NucleusChromosomeCytoplasmWhen TrimethylatedLocalizes In The Cytoplasm |
| Alternative Antibody Names | Anti-X-Ray Repair Cross-Complementing Protein 6 antibodyAnti-5'-Deoxyribose-5-Phosphate Lyase Ku70 antibodyAnti-5'-Drp Lyase Ku70 antibodyAnti-70 Kda Subunit Of Ku Antigen antibodyAnti-Atp-Dependent Dna Helicase 2 Subunit 1 antibodyAnti-Atp-Dependent Dna Helicase Ii 70 Kda Subunit antibodyAnti-Ctc Box-Binding Factor 75 Kda Subunit antibodyAnti-Ctc75 antibodyAnti-Ctcbf antibodyAnti-Dna Repair Protein Xrcc6 antibodyAnti-Lupus Ku Autoantigen Protein P70 antibodyAnti-Ku70 antibodyAnti-Thyroid-Lupus Autoantigen antibodyAnti-Tlaa antibodyAnti-X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 antibodyAnti-XRCC6 antibodyAnti-G22P1 antibody |
Information sourced from Uniprot.org