| Short Description : | Rabbit polyclonal anti-Acetyl-Transcription factor NF-E4-Lys43 for use in WB, ELISA and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA/IHC |
| Host: | Rabbit |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NFE4Uniprot ID=NFE4_HUMAN" |
| Immunogen: | Synthesized acetyl-peptide derived from the human NF-E4 around the acetylation site of K43. |
| Specificity: | Acetyl-NF-E4 (K43) Polyclonal AntibodySynthesized peptide derived from the human NF-E4 around the acetylation site of K43. |
| Post Translational Modifications | Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor at the gamma-promoter. Ubiquitinated.leading to its degradation by the proteasome. Acetylation at Lys-43 prevents ubiquitination. |
| Function | Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter. |
| Protein Name | Transcription Factor Nf-E4 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Nf-E4 antibodyAnti-NFE4 antibody |
Information sourced from Uniprot.org
