||WB, IHC-P, IF-P, ELISA
||Human, Mouse, Rat
||FOR RESEARCH USE ONLY (RUO).
||Rabbit polyclonal antibody anti-Acetyl-Heat Shock Protein Hsp 90-Alpha-Lys292/284 (251-300) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
||PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
||The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
||WB 1:500-1:2000IHC 1:100-300ELISA 1:20000
||Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
||Acetyl-HSP90AA1-Lys292/284 polyclonal antibody (Heat Shock Protein Hsp 90-Alpha) binds to endogenous Heat Shock Protein Hsp 90-Alpha at the amino acid region 251-300.
||The antiserum was produced against synthesized Acetyl-peptide derived from human HSP90A/B around the Acetylation site of Lys292/284 at amino acid range 251-300
| Function || Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochodria outer membrane which promotes host antiviral response. |
| Protein Name || Heat Shock Protein Hsp 90-AlphaHeat Shock 86 KdaHsp 86Hsp86Lipopolysaccharide-Associated Protein 2Lap-2Lps-Associated Protein 2Renal Carcinoma Antigen Ny-Ren-38 |
| Database Links || Reactome: R-HSA-1227986Reactome: R-HSA-1236382Reactome: R-HSA-1474151Reactome: R-HSA-168928Reactome: R-HSA-192905Reactome: R-HSA-2029482Reactome: R-HSA-203615Reactome: R-HSA-2565942Reactome: R-HSA-3000484Reactome: R-HSA-3371497Reactome: R-HSA-3371511Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-399954Reactome: R-HSA-4420097Reactome: R-HSA-5218920Reactome: R-HSA-5336415Reactome: R-HSA-5601884Reactome: R-HSA-5620912Reactome: R-HSA-5637810Reactome: R-HSA-5675482Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-8852276Reactome: R-HSA-8854518Reactome: R-HSA-8863795Reactome: R-HSA-8939211Reactome: R-HSA-9009391Reactome: R-HSA-9013418Reactome: R-HSA-9018519Reactome: R-HSA-9613829Reactome: R-HSA-9634285Reactome: R-HSA-9646399Reactome: R-HSA-9652282Reactome: R-HSA-9664565Reactome: R-HSA-9665233Reactome: R-HSA-9665244Reactome: R-HSA-9665245Reactome: R-HSA-9665246Reactome: R-HSA-9665247Reactome: R-HSA-9665249Reactome: R-HSA-9665250Reactome: R-HSA-9665251Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9665737Reactome: R-HSA-9679191 |
| Cellular Localisation || NucleusCytoplasmMelanosomeCell MembraneMitochondrionIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names || Anti-Heat Shock Protein Hsp 90-Alpha antibodyAnti-Heat Shock 86 Kda antibodyAnti-Hsp 86 antibodyAnti-Hsp86 antibodyAnti-Lipopolysaccharide-Associated Protein 2 antibodyAnti-Lap-2 antibodyAnti-Lps-Associated Protein 2 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-38 antibodyAnti-HSP90AA1 antibodyAnti-HSP90A antibodyAnti-HSPC1 antibodyAnti-HSPCA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance