| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA/ChIP |
| Reactivity: | Human/Mouse/Rat/Other |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. ChIP:5 Mu g antibody for 5 Mu g-10 Mu g of Chromatin |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4C16 |
| Gene ID: | 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370 |
| Uniprot ID: | H4_HUMAN |
| Immunogen Region: | 1-13 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_001029249.1). |
| Immunogen Sequence: | MSGRGKGGKGLGKGGAKRHR KVLRDNIQGITKPAIRRLAR RGGVKRISGLIYEETRGVLK VFLENVIRDAVTYTEHAKRK TVTAMDVVYALKRQGRTLYG |
| Post Translational Modifications | Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by KMT5A/SET8. Dimethylation and trimethylation is performed by KMT5B and KMT5C and induces gene silencing. Monomethylated at Lys-13 (H4K12me1) by N6AMT1.H4K12me1 modification is present at the promoters of numerous genes encoding cell cycle regulators. Acetyl-methylated at Lys-6 and Lys-13 (H4K5acme and H4K12acme, respectively), acetyl-methylation is an epigenetic mark of active chromatin associated with increased transcriptional initiation. Acetyl-methylation is formed by acetylation by EP300/p300 of lysine residues that are already monomethylated on the same side chain. H4K5acme and H4K12acme marks specifically bind BRD2. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ufmylated.monofmylated by UFL1 at Lys-32 (H4K31Ufm1) in response to DNA damage. Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Butyrylation of histones marks active promoters and competes with histone acetylation. Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. Delactylated by SIRT3 at Lys-17 (H4K16la). |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Protein Name | Histone H4 |
| Database Links | Reactome: R-HSA-110328 Reactome: R-HSA-110329 Reactome: R-HSA-110330 Reactome: R-HSA-110331 Reactome: R-HSA-1221632 Reactome: R-HSA-171306 Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-3214815 Reactome: R-HSA-3214841 Reactome: R-HSA-3214842 Reactome: R-HSA-3214847 Reactome: R-HSA-3214858 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-4551638 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-606279 Reactome: R-HSA-68616 Reactome: R-HSA-69473 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379 Reactome: R-HSA-9616222 Reactome: R-HSA-9670095 Reactome: R-HSA-9710421 Reactome: R-HSA-977225 Reactome: R-HSA-9821002 Reactome: R-HSA-9821993 Reactome: R-HSA-9843940 Reactome: R-HSA-9843970 Reactome: R-HSA-9845323 |
| Cellular Localisation | Nucleus Chromosome |
| Alternative Antibody Names | Anti-Histone H4 antibody Anti-H4C1 antibody Anti-H4 antibody Anti-A antibody Anti-H4FA antibody Anti-HIST1H4A.H4C2 antibody Anti-H4 antibody Anti-I antibody Anti-H4FI antibody Anti-HIST1H4B.H4C3 antibody Anti-H4 antibody Anti-G antibody Anti-H4FG antibody Anti-HIST1H4C.H4C4 antibody Anti-H4 antibody Anti-B antibody Anti-H4FB antibody Anti-HIST1H4D.H4C5 antibody Anti-H4 antibody Anti-J antibody Anti-H4FJ antibody Anti-HIST1H4E.H4C6 antibody Anti-H4 antibody Anti-C antibody Anti-H4FC antibody Anti-HIST1H4F.H4C8 antibody Anti-H4 antibody Anti-H antibody Anti-H4FH antibody Anti-HIST1H4H.H4C9 antibody Anti-H4 antibody Anti-M antibody Anti-H4FM antibody Anti-HIST1H4I.H4C11 antibody Anti-H4 antibody Anti-E antibody Anti-H4FE antibody Anti-HIST1H4J.H4C12 antibody Anti-H4 antibody Anti-D antibody Anti-H4FD antibody Anti-HIST1H4K.H4C13 antibody Anti-H4 antibody Anti-K antibody Anti-H4FK antibody Anti-HIST1H4L.H4C14 antibody Anti-H4 antibody Anti-N antibody Anti-H4F2 antibody Anti-H4FN antibody Anti-HIST2H4 antibody Anti-HIST2H4A.H4C15 antibody Anti-H4 antibody Anti-O antibody Anti-H4FO antibody Anti-HIST2H4B.H4C16 antibody Anti-H4-16 antibody Anti-HIST4H4 antibody |
Information sourced from Uniprot.org
