• Immunofluorescence analysis of HeLa cells using acetyl-ACC1 antibody (STJ118055) at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells using acetyl-ACC1 antibody (STJ118055) at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using acetyl-ACC1 antibody (STJ118055) at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using acetyl-ACC1 antibody (STJ118055) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-acetyl-CoA carboxylase alpha antibody (STJ118055)

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STJ118055
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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against ACACA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IF: 1:50-1:200
Uniprot ID: ACACA_HUMAN
Gene ID: 31
Gene Symbol: ACACA
Immunogen: A synthetic peptide of human acetyl-CoA carboxylase alpha.
Background Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Tissue Specificity Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues.expressed at low level in pulmonary tissue.not detected in the liver.
Post Translational Modifications Phosphorylation on Ser-1263 is required for interaction with BRCA1. Phosphorylation at Ser-80 by AMPK inactivates enzyme activity. The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Function Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Database Links Reactome: R-HSA-163765
Cellular Localisation Cytoplasm
Cytosol
Protein Name Acetyl-Coa Carboxylase 1
Acc1
Acetyl-Coenzyme A Carboxylase Alpha
Acc-Alpha
Alternative Names Anti-Acetyl-Coa Carboxylase 1 antibody
Anti-Acc1 antibody
Anti-Acetyl-Coenzyme A Carboxylase Alpha antibody
Anti-Acc-Alpha antibody
Anti-ACACA antibody
Anti-ACAC antibody
Anti-ACC1 antibody
Anti-ACCA antibody

Information sourced from uniprot.org

6 months standard