Anti-ACER1 antibody (100-180 aa) (STJ97625)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from ASAH3 at the amino acid range 100-180
STJ97625
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General Information

Short DescriptionRabbit polyclonal anti-Alkaline ceramidase 1 (100-180 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
ELISA 1:10000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolACER1
Gene ID125981
Uniprot IDACER1_HUMAN
ImmunogenSynthesized peptide derived from ASAH3 at the amino acid range 100-180
Immunogen Region100-180 aa
SpecificityASAH3 Polyclonal Antibody detects endogenous levels of ASAH3 protein.

Additional Info

Function Endoplasmic reticulum ceramidase that catalyzes the hydrolysis of ceramides into sphingosine and free fatty acids at alkaline pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Exhibits a strong substrate specificity towards the natural stereoisomer of ceramides with D-erythro-sphingosine as a backbone and has a higher activity towards very long-chain unsaturated fatty acids like the C24:1-ceramide. May also hydrolyze dihydroceramides to produce dihydrosphingosine. ACER1 is a skin-specific ceramidase that regulates the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes and more generally plays an important role in skin homeostasis.
Protein Name Alkaline Ceramidase 1
Alkcdase 1
Alkaline Cdase 1
Acylsphingosine Deacylase 3
N-Acylsphingosine Amidohydrolase 3
Database Links Reactome: R-HSA-9845614
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Alkaline Ceramidase 1 antibody
Anti-Alkcdase 1 antibody
Anti-Alkaline Cdase 1 antibody
Anti-Acylsphingosine Deacylase 3 antibody
Anti-N-Acylsphingosine Amidohydrolase 3 antibody
Anti-ACER1 antibody
Anti-ASAH3 antibody

Information sourced from Uniprot.org

Citations

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