| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ACADS |
| Gene ID: | 35 |
| Uniprot ID: | ACADS_HUMAN |
| Immunogen Region: | 1-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1). |
| Immunogen Sequence: | MAAALLARASGPARRALCPR AWRQLHTIYQSVELPETHQM LLQTCRDFAEKELFPIAAQV DKEHLFPAAQVKKMGGLGLL AMDVPEELGGAGLDYLAYAI AMEEISRGCASTGVIMSVNN SLYLGPILKFGSKEQKQAWV TPFTSGDKIGCFALSEPGNG SDAGAASTTARAEGDSWVLN GTKAWITNAWEASAAVVFAS TDRALQNKGISAFLVPMPTP GLTLGKKEDKLGIRGSSTA |
| Function | Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains. |
| Protein Name | Short-Chain Specific Acyl-Coa Dehydrogenase - MitochondrialScadButyryl-Coa Dehydrogenase |
| Database Links | Reactome: R-HSA-77350Reactome: R-HSA-77352 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Short-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibodyAnti-Scad antibodyAnti-Butyryl-Coa Dehydrogenase antibodyAnti-ACADS antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
