• Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g ACADS antibody (STJ29310). Western blot was performed from the immunoprecipitate using ACADS antibody (STJ29310) at a dilution of 1:1000.
  • Western blot analysis of various lysates using ACADS Rabbit polyclonal antibody (STJ29310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-ACADS antibody (1-260) (STJ29310)

SKU:
STJ29310

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACADS (1-260) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACADS
Gene ID: 35
Uniprot ID: ACADS_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).
Immunogen Sequence: MAAALLARASGPARRALCPR AWRQLHTIYQSVELPETHQM LLQTCRDFAEKELFPIAAQV DKEHLFPAAQVKKMGGLGLL AMDVPEELGGAGLDYLAYAI AMEEISRGCASTGVIMSVNN SLYLGPILKFGSKEQKQAWV TPFTSGDKIGCFALSEPGNG SDAGAASTTARAEGDSWVLN GTKAWITNAWEASAAVVFAS TDRALQNKGISAFLVPMPTP GLTLGKKEDKLGIRGSSTA
Function Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.
Protein Name Short-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial
Scad
Butyryl-Coa Dehydrogenase
Database Links Reactome: R-HSA-77350
Reactome: R-HSA-77352
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Short-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibody
Anti-Scad antibody
Anti-Butyryl-Coa Dehydrogenase antibody
Anti-ACADS antibody

Information sourced from Uniprot.org

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