| Host: | Rabbit |
| Applications: | IF/WB/IHC/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Broad substrate specificity ATP-binding cassette transporter ABCG2 (461-510 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IF 1:50-200 WB 1:500-1:2000 IHC-P 1:100-1:300 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ABCG2 |
| Gene ID: | 9429 |
| Uniprot ID: | ABCG2_HUMAN |
| Immunogen Region: | 461-510 aa |
| Specificity: | ABCG2 Polyclonal Antibody detects endogenous levels of ABCG2 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human ABCG2 at the amino acid range 461-510 |
| Post Translational Modifications | N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional. Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter. |
| Function | Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate. Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk. Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability. Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus. May play a role in early stem cell self-renewal by blocking differentiation. |
| Protein Name | Broad Substrate Specificity Atp-Binding Cassette Transporter Abcg2 Atp-Binding Cassette Sub-Family G Member 2 Breast Cancer Resistance Protein Cdw338 Mitoxantrone Resistance-Associated Protein Placenta-Specific Atp-Binding Cassette Transporter Urate Exporter Cd Antigen Cd338 |
| Database Links | Reactome: R-HSA-1660661 Reactome: R-HSA-189451 Reactome: R-HSA-189483 Reactome: R-HSA-2161517 Reactome: R-HSA-917937 Reactome: R-HSA-9725554 Reactome: R-HSA-9753281 Reactome: R-HSA-9793528 Reactome: R-HSA-9818032 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Apical Cell Membrane Mitochondrion Membrane Enriched In Membrane Lipid Rafts |
| Alternative Antibody Names | Anti-Broad Substrate Specificity Atp-Binding Cassette Transporter Abcg2 antibody Anti-Atp-Binding Cassette Sub-Family G Member 2 antibody Anti-Breast Cancer Resistance Protein antibody Anti-Cdw338 antibody Anti-Mitoxantrone Resistance-Associated Protein antibody Anti-Placenta-Specific Atp-Binding Cassette Transporter antibody Anti-Urate Exporter antibody Anti-Cd Antigen Cd338 antibody Anti-ABCG2 antibody Anti-ABCP antibody Anti-BCRP antibody Anti-BCRP1 antibody Anti-MXR antibody |
Information sourced from Uniprot.org