• Western blot analysis of HeLa, using MRP4/ABCC4 Rabbit polyclonal antibody (STJ11104283) at 1:1000 dilution. membrane protein extract isolated from HelaSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ABCC4 antibody (1067-1325) (STJ11104283)

SKU:
STJ11104283

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Atp-Binding Cassette Sub-Family C Member 4 (1067-1325) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCC4
Gene ID: 10257
Uniprot ID: MRP4_HUMAN
Immunogen Region: 1067-1325
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1067-1325 of human MRP4/ABCC4 (NP_005836.2).
Immunogen Sequence: SQEKVGIVGRTGAGKSSLIS ALFRLSEPEGKIWIDKILTT EIGLHDLRKKMSIIPQEPVL FTGTMRKNLDPFNEHTDEEL WNALQEVQLKETIEDLPGKM DTELAESGSNFSVGQRQLVC LARAILRKNQILIIDEATAN VDPRTDELIQKKIREKFAHC TVLTIAHRLNTIIDSDKIMV LDSGRLKEYDEPYVLLQNKE SLFYKMVQQLGKAEAAALTE TAKQVYFKRNYPHIGHTDH
Tissue Specificity Widely expressed, with particularly high levels in prostate, but is barely detectable in liver. sinusoidal membrane of hepatocytes.
Post Translational Modifications N-glycosylated.leading to substrate-selective effects on its transport activity.
Function ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins. Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4. Mediates the cotransport of bile acids with reduced glutathione (GSH). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules. Confers resistance to anticancer agents such as methotrexate.
Protein Name Atp-Binding Cassette Sub-Family C Member 4
Mrp/Cmoat-Related Abc Transporter
Multi-Specific Organic Anion Transporter B
Moat-B
Multidrug Resistance-Associated Protein 4
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-382556
Reactome: R-HSA-9748787
Reactome: R-HSA-9753281
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Its Localization To The Basolateral Or Apical Membranes Is Tissue-Dependent
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family C Member 4 antibody
Anti-Mrp/Cmoat-Related Abc Transporter antibody
Anti-Multi-Specific Organic Anion Transporter B antibody
Anti-Moat-B antibody
Anti-Multidrug Resistance-Associated Protein 4 antibody
Anti-ABCC4 antibody
Anti-MOATB antibody
Anti-MRP4 antibody

Information sourced from Uniprot.org

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