• Western blot analysis of lysates from wild type (WT) and MRP4/ABCC4 knockdown (KD) 293T cells, using [KD Validated] MRP4/ABCC4 Rabbit monoclonal antibody (STJ11104887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates, using [KD Validated] MRP4/ABCC4 Rabbit monoclonal antibody (STJ11104887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ABCC4 antibody (1067-1325) [S4887RM] (STJ11104887)

SKU:
STJ11104887

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Atp-Binding Cassette Sub-Family C Member 4 (1067-1325) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4887RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCC4
Gene ID: 10257
Uniprot ID: MRP4_HUMAN
Immunogen Region: 1067-1325
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1067-1325 of human MRP4/ABCC4. (NP_005836.2).
Tissue Specificity Widely expressed, with particularly high levels in prostate, but is barely detectable in liver. sinusoidal membrane of hepatocytes.
Post Translational Modifications N-glycosylated.leading to substrate-selective effects on its transport activity.
Function ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins. Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4. Mediates the cotransport of bile acids with reduced glutathione (GSH). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules. Confers resistance to anticancer agents such as methotrexate.
Protein Name Atp-Binding Cassette Sub-Family C Member 4
Mrp/Cmoat-Related Abc Transporter
Multi-Specific Organic Anion Transporter B
Moat-B
Multidrug Resistance-Associated Protein 4
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-382556
Reactome: R-HSA-9748787
Reactome: R-HSA-9753281
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Its Localization To The Basolateral Or Apical Membranes Is Tissue-Dependent
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family C Member 4 antibody
Anti-Mrp/Cmoat-Related Abc Transporter antibody
Anti-Multi-Specific Organic Anion Transporter B antibody
Anti-Moat-B antibody
Anti-Multidrug Resistance-Associated Protein 4 antibody
Anti-ABCC4 antibody
Anti-MOATB antibody
Anti-MRP4 antibody

Information sourced from Uniprot.org

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