• Immunofluorescence analysis of HeLa cells using ABCB7 Rabbit polyclonal antibody (STJ116899) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ABCB7 in paraffin-embedded mouse brain using ABCB7 Rabbit polyclonal antibody (STJ116899) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ABCB7 in paraffin-embedded human gastric cancer using ABCB7 Rabbit polyclonal antibody (STJ116899) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ABCB7 in paraffin-embedded rat heart using ABCB7 Rabbit polyclonal antibody (STJ116899) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ABCB7 Rabbit polyclonal antibody (STJ116899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ABCB7 antibody (503-753) (STJ116899)

SKU:
STJ116899

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCB7 (503-753) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCB7
Gene ID: 22
Uniprot ID: ABCB7_HUMAN
Immunogen Region: 503-753
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 503-753 of human ABCB7 (NP_004290.2).
Immunogen Sequence: AIVGGSGSGKSTIVRLLFRF YEPQKGSIYLAGQNIQDVSL ESLRRAVGVVPQDAVLFHNT IYYNLLYGNISASPEEVYAV AKLAGLHDAILRMPHGYDTQ VGERGLKLSGGEKQRVAIAR AILKDPPVILYDEATSSLDS ITEETILGAMKDVVKHRTSI FIAHRLSTVVDADEIIVLDQ GKVAERGTHHGLLANPHSIY SEMWHTQSSRVQNHDNPKWE AKKENISKEEERKKLQEEI
Function Exports glutathione-coordinated iron-sulfur clusters such as 2Fe-2S-(GS)4 cluster from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur (Fe/S) cluster-containing proteins and participates in iron homeostasis. Moreover, through a functional complex formed of ABCB7, FECH and ABCB10, also plays a role in the cellular iron homeostasis, mitochondrial function and heme biosynthesis. In cardiomyocytes, regulates cellular iron homeostasis and cellular reactive oxygen species (ROS) levels through its interaction with COX4I1. May also play a role in hematopoiesis.
Protein Name Iron-Sulfur Clusters Transporter Abcb7 - Mitochondrial
Atp-Binding Cassette Sub-Family B Member 7 - Mitochondrial
Atp-Binding Cassette Transporter 7
Abc Transporter 7 Protein
Database Links Reactome: R-HSA-1369007
Reactome: R-HSA-2564830
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Iron-Sulfur Clusters Transporter Abcb7 - Mitochondrial antibody
Anti-Atp-Binding Cassette Sub-Family B Member 7 - Mitochondrial antibody
Anti-Atp-Binding Cassette Transporter 7 antibody
Anti-Abc Transporter 7 Protein antibody
Anti-ABCB7 antibody
Anti-ABC7 antibody

Information sourced from Uniprot.org

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